Transportation conditions : normal temperature transportation
Item: 51019: 50 reactions
51020: 100 reactions
product description
BIOG cfDNA Easy Kit is a kit specially developed by Changzhou Baidai Biotechnology Co., Ltd. to extract free DNA such as serum and plasma. The BIOG cfDNA Easy Kit uses the latest high-quality imported ion membranes, and the lysate and eluent are optimized several times to efficiently separate DNA. Compared with other brands, the extracted DNA has higher yield, higher purity, and maximizes the removal of impurities such as proteins, pigments, lipids, etc., and can be directly applied to PCR, real-time PCR and various enzyme digestion tests. .
Features
â—Ž Easy and fast operation, the ideal DNA can be obtained within half an hour;
â—ŽThe extracted DNA has high purity and no inhibitor, and the A260/A280 is 1.7-1.9;
â—ŽHigher output, 20% higher than domestic similar products;
â—Ž can be used for DNA extraction in free samples such as serum and plasma;
â—Ž It is free of toxic solvents such as phenol and chloroform, safe and non-toxic.
Kit composition
Component | 50 times | 100 times |
Adsorption column and collection tube | 50 each | 100 each |
Lysate | 18mL | 36 mL |
Washing liquid A | 21mL | 42 mL |
Washing liquid B | 9 mL | 18 mL |
Eluent | 3mL | 6 mL |
Digestive juice | 1.2 mL | 2.4 mL |
DNA Carrier | 0.24 mL | 0.48 mL |
Instruction manual | 1 copy | 1 copy |
Steps
1. Please prepare yourself: absolute ethanol, 1.5mL centrifuge tube.
2. Remove the washing solution and do the following:
a) Washing solution A: 21 mL was added to 9 mL of absolute ethanol; 42 mL was added to 18 mL of absolute ethanol.
b) Washing solution B: 9 mL was added to 21 mL of absolute ethanol; 18 mL was added to 42 mL of absolute ethanol.
c) If the prepared precipitate and washing solution are precipitated, they can be dissolved at 37 ° C, shaken and used.
3. Take a 1.5 mL centrifuge tube, add 200 μL of sample, mix 4 μL of DNA Carrier, add 300 μL of lysate and 20 μL of digestive juice, mix by shaking, and bath at 56 ° C for 10 minutes.
4. Add 1000 μL of absolute ethanol and mix gently by inversion. If there is a semi-transparent suspension, it will not affect the DNA extraction and subsequent experiments.
5. Place the adsorption column into the collection tube, transfer 760 μL of the above solution into the adsorption column, let stand for 2 minutes, centrifuge at 12,000 rpm for 4 minutes at 4 ° C, and discard the waste liquid in the collection tube;
6. Return the column to the collection tube and transfer the remaining 760 μL of the solution to the column. Repeat step 5.
7. Place the adsorption column back into the collection tube, add 500 μL of Wash A to the adsorption column, centrifuge at 12,000 rpm for 4 minutes at 4 ° C, and discard the waste liquid in the collection tube.
8. Put the adsorption column back into the collection tube, add 500 μL of washing solution B to the adsorption column, centrifuge at 12,000 rpm for 4 minutes at 4 ° C, and discard the waste liquid in the collection tube.
9. Place the column back into the collection tube and centrifuge at 12,000 rpm for 4 minutes at 4 ° C to remove any remaining wash solution.
10. Remove the adsorption column, place it in a new 1.5 mL centrifuge tube, add 30-50 μL of eluate, let stand for 3 minutes, centrifuge at 12,000 rpm for 4 minutes at 4 ° C, and collect the DNA solution. The extracted DNA can be used in the next experiment or at -20 °C.
Precautions:
Lysates, precipitates, and washings contain irritating chemicals. Take precautions during operation to avoid direct contact with the skin and prevent inhalation of nose and mouth. If you accidentally get into skin or eyes, rinse immediately with water or saline. Seek medical attention if necessary.
Storage, transportation and expiration date
This kit can be transported at room temperature, stored at room temperature (15-25 °C), valid for 12 months. Please store the digestive juice and DNA carrier at -20 °C to avoid repeated freezing and thawing.
QC
This product has passed strict quality inspection and proved that there is no biological pollution.
Note : This product is for scientific research only.
Changzhou Baidai Biotechnology Co., Ltd.
Address: New Oriental Logistics Park, No. 51 Dongfang East Road, Changzhou Tel: 0519-88050392 Fax: 0519-83382790
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