At present, there are two kinds of inoculation methods for mushroom fungus rods: one is the inoculation box method and the other is the open inoculation method. The inoculation box method has the advantages of high rate of inoculation, stable effect, and less restrictions. The disadvantage is that the speed is slow, the inoculation volume per person per hour is 30-40 bags, and the open inoculation method has the advantages of high inoculation speed and high work efficiency. The number of hours of inoculation in the 60-90 bags, inoculation than the inoculation box to increase 1-3 times, the disadvantage is that the technical requirements are higher, otherwise the effect is not stable enough, followed by a large amount of sterilization drugs. The inoculation kit method has been used for many years. Only the open inoculation method is described here:
This method is a kind of practical inoculation method which has been improved in recent years for the increase of the number of single-planted plants.
(1) Cooling sites for rod cooling and open inoculation method are inoculation sites. Therefore, the cooling site must be relatively sealed. The environment with good hygienic conditions should not exceed 50 square meters. If the space is too large, tents should be hung. (2 m 2 m 2 m film account made of 8 silk agricultural film), the sterilized rods are moved into the inoculation chamber, the quantity is 1000-2500 bags, and during the cooling process, the material rods are not kept in the cooling process. Affected or less affected by dirty air outside.
(2) Disinfection Place bacteria and other objects on rods, then ignite them with aerosol disinfectant 4-5 boxes (160-200 g), and cover the rods tightly with film. Try not to let the aerosol disinfectant The smoke escapes and the disinfection time is 3-6 hours.
(3) Open inoculation before inoculation Open the inoculum first and open the door. Inoculated with a plastic shed, you can open the account door, and then open the film covering the rod, until the vaccination personnel can tolerate it. Can be inoculated. Open the door during inoculation to reduce the increase in room temperature. The strain pretreatment and inoculation method are the same as the inoculation box.
(4) Management after inoculation For the use of plastic film sealing or strain sealing (also called not sealing) without using bagging, all kinds of residual debris should be cleaned, and the film is re-covered in the bacterial rod stack after the dirty air is exhausted. The membrane is lifted once a day in the early morning or at night. After 5-7 days of bacterial colonization, the bacteria can be removed from the membrane or turned to the awning. Practice has shown that doing so can greatly increase the survival rate.
This method is a kind of practical inoculation method which has been improved in recent years for the increase of the number of single-planted plants.
(1) Cooling sites for rod cooling and open inoculation method are inoculation sites. Therefore, the cooling site must be relatively sealed. The environment with good hygienic conditions should not exceed 50 square meters. If the space is too large, tents should be hung. (2 m 2 m 2 m film account made of 8 silk agricultural film), the sterilized rods are moved into the inoculation chamber, the quantity is 1000-2500 bags, and during the cooling process, the material rods are not kept in the cooling process. Affected or less affected by dirty air outside.
(2) Disinfection Place bacteria and other objects on rods, then ignite them with aerosol disinfectant 4-5 boxes (160-200 g), and cover the rods tightly with film. Try not to let the aerosol disinfectant The smoke escapes and the disinfection time is 3-6 hours.
(3) Open inoculation before inoculation Open the inoculum first and open the door. Inoculated with a plastic shed, you can open the account door, and then open the film covering the rod, until the vaccination personnel can tolerate it. Can be inoculated. Open the door during inoculation to reduce the increase in room temperature. The strain pretreatment and inoculation method are the same as the inoculation box.
(4) Management after inoculation For the use of plastic film sealing or strain sealing (also called not sealing) without using bagging, all kinds of residual debris should be cleaned, and the film is re-covered in the bacterial rod stack after the dirty air is exhausted. The membrane is lifted once a day in the early morning or at night. After 5-7 days of bacterial colonization, the bacteria can be removed from the membrane or turned to the awning. Practice has shown that doing so can greatly increase the survival rate.
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