Introduction <br>The regulatory standards for biopharmaceutical approvals are constantly changing. Regulatory regulations for the monoclonal source of cell lines have driven us to use more efficient techniques or methods in biopharmaceutical development. Many researchers typically use imaging systems (such as CloneSelect Imager) to verify the growth of monoclonal and monitored cells in liquid media. In fact, the CloneSelect Imager system can also image cells in semi-solid media. The location of the cells in the semi-solid medium is relatively fixed, allowing researchers to more accurately image the same cell at different time points. Conventional methods in the laboratory typically use limited dilution for cell cloning. However, this method uses liquid media to culture cells, making tracking cells difficult or less accurate. Because of the ease of movement of the suspended cells within the well during processing of the microplate (Figure 1). It is not possible to ensure that the same cell is imaged at different time points, thus greatly reducing the accuracy of monoclonal validation. In addition, limited dilution has the potential to add multiple cells in one well, requiring additional multiple subclones to increase the probability of monoclonal. The use of semi-solid medium to culture cells provides researchers with a faster and simpler method of cloning and is useful for tracking cell growth. The position of the cells in the semi-solid medium is relatively constant, and the cell position does not move during routine experimental procedures and can be grown into individual cell clones. Therefore, multiple cells can be planted in the same well, and imaging at different time points can clearly track the growth of individual cells and provide monoclonal digital verification data. CloneSelect Imager provides researchers with a way to image cells in semi-solid media. This system uses a non-invasive assay that allows for accurate quantification of cell growth without the need for labeled cells for direct imaging; single-cell source cloning validation ensures that only stable monoclonals are selected for downstream studies. In this paper, the performance of CloneSelect Imager in suspension CHO-s cells in CloneMediaTM CHO Growth A semi-solid medium was verified by actual experiments. This semi-solid medium component is specifically optimized for growth in a variety of CHO cell lines.
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