Chinese and American medical experts work together to achieve new progress in 3D bioprinting

On November 16th, the medical network injected various functional cells into the printer to accurately construct the multi-layer cavity organization. Is this scientific or realistic? Renji Hospital, affiliated to Shanghai Jiaotong University School of Medicine, revealed on the 15th that the research of Dr. Pei Qingmeng of the Department of Plastic Surgery revealed that 3D bioprinting has achieved cavity tissue printing and that cells can survive for a long time after printing.

Pi Qingmeng's research is expected to be used to achieve accurate construction of complex cavity tissues or organs, especially for patients requiring cavity organ or tissue transplantation, a new form of donor access. The research results are also expected to be used in many fields such as in vitro vascular, intestinal, urinary system and other hollow organ disease model simulation, drug screening, tissue transplantation substitutes and the like.

The international biomaterials magazine "Advanced Materials" published a research paper entitled "Digital Adjustable Microfluidic Bioprinting of Multilayer Ring Organization" online. It is understood that Professor Yu Shrike Zhang of Harvard Medical School and Professor Ali Khademhoseini of UCLA are co-authors. This research means that China's 3D bio-printing technology is gradually aligning with the world.

Where is 3D bioprinting difficult? According to Pi Qingmeng, bioprinting needs to consider a lot of factors, such as the cytocompatibility and mechanical strength of printed materials. Compared with general solid tissues, cavity tissue construction is more complicated, requiring not only multi-cellular components, but also different sub-layer cell types and functions. The research confirmed that 3D bio-printing can quickly, accurately and individually construct multi-layered cavity tissues such as blood vessels and urethra containing different functional cells, and the tissue structure is clear.

Dr. Pi Qingmeng said that printing solid tissue is equivalent to baking a solid bread, printing a multi-layered hollow tissue, which is equivalent to baking a hollow bread. The hollow is divided into different layers, and the layers are tightly fitted. Separate from each other. Compared with general 3D printing, biological printing requires the whole process to control cell viability and protect cells from damage. Secondly, different functional cells in the cavity can be evenly distributed at all levels, which is more in line with the normal structure of the human body.

The study also confirmed that the control system can realize the idea of ​​repeated switching of single-layer structure and double-layer structure in the same lumen structure. After vascular cells and urethral cells were mixed with the composite hydrogel, the multi-layered luminal tissue was printed by MCCES. The in vitro culture showed that the cell viability was over 80%, and the cells could spread and grow on the hydrogel scaffold material. Specific markers.