Laboratory management practices
The artificial insemination station laboratory is a place for semen inspection, treatment and storage. In order to produce high-quality sperm that meets the insemination requirements, it must be of good quality to ensure that the vitality of each bottle of semen from the station is not less than 0.7, 72. The preservation rate is not affected within an hour. The following regulations are made for the daily operation of the laboratory.
1. The laboratory requires cleanliness, cleanliness, hygiene, and thorough cleaning once a week;
2. Non-laboratory personnel are not allowed to enter the laboratory under normal circumstances, and sperm extractors are not allowed to enter the laboratory;
3, all equipment and equipment should be carefully read the instructions, by a person in accordance with the operating procedures used and maintained; in particular, high-pressure steam sterilizer, ultrasonic washing device, double steamer should pay attention to personal safety when using;
4, a variety of electrical equipment should be selected to adapt to the socket, in addition to refrigerators, semen preserver, constant temperature incubator, etc., general electrical appliances require people to go off, when no drying oven set temperature should not exceed 100 °C;
5. Cleaning and disinfection of articles and utensils: All utensils should be washed with detergent or washing powder, rinsed with distilled water, dried at 60°C (drying temperature of glassware can be higher than 100°C), and wrapped with tin foil. The glassware was dried at a temperature of 180°C for 1 hour, and non-heat-resistant dishes and utensils were sterilized by autoclaving at 121°C for 20 minutes.
6. The preparation of the diluent, semen check, dilution and packing must be performed in accordance with the artificial insemination operating procedures;
7, laboratory equipment and equipment to keep clean and hygienic: the laboratory equipment used in the laboratory, such as microscopes, drying oven, water bath, 17 °C semen preservation box, refrigerator, 37 °C constant temperature plate, electronic day equality, will inevitably keep clean, microscope The lens (eyepiece and objective) should be soaked with xylene every 2 weeks to keep it clean;
8. The side windows of the transfer port between the sperm collection room and the laboratory can only be opened and used in sequence when delivering items;
9, laboratory floor, test bench to keep clean and clean;
10. Before leaving the laboratory after work, be sure to check whether the power supply, faucet, door, and window are closed, so that you can leave the laboratory without fail to ensure safety;
11, fire and security, pay attention to safety.
Acquisition procedures
1. The extractor carries double gloves in one hand, and the other holds a 37°C flask (containing a disposable food bag) to collect semen;
2. The breeder rushes the boars to be refined to the collecting column, cleans their abdomen and foreskin with 0.1% potassium permanganate solution, and then cleans them with warm water (in summer tap water) to avoid drug residue damage to the sperm;
3, the extractor extrudes the circumcision of the boar for the foreskin and massages the foreskin of the boar to stimulate the animal to climb over the counterfeit table;
4. The boar climbs over the fake table and gradually extends the penis, removes the outer gloves, and introduces the boar's penis into the empty fist;
5, hand (the thumb and the glans in the opposite direction) grasping out of the boar penis spiral penis, along the forward momentum of the penis "S'' shape bending straight, grip the penis glans to prevent its rotation, public Pigs can ejaculate;
6. Use a four-layer gauze filter to collect concentrated semen in a disposable food pouch inside the thermos cup. The small amount of semen initially injected contains very few sperm and can be taken without having to receive it. Some boar concentrate the semen in 2-3 stages. Shooting until the boar ejaculation is complete, the ejaculation process lasts 5-7 minutes;
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