experimental method 1) Animals were acclimated to the experimental environment for 1 week, weighed, and fasted for 24 hours. Thereafter, after the end of the eight-arm maze training, the normal foodstuffs (16-20 g of rats, 2-3 g of mice according to body weight) were used to maintain the body weight of 80%-85% of the normal fed rats.
2) The next day, the arms and the center of the labyrinth are separated by food particles (4 to 5 each, about 3 to 4 mm in diameter). Then, at the same time, 4 animals were placed in the middle of the maze (the door leading to each arm was opened). Let it freely ingest and explore for 10 minutes. Â
3) On the third day, repeat the training for the next day. This process allows animals to become familiar with the maze environment without strong stress.
4) From the fourth day, the animals will be trained individually: put a grain of food at each of the arms near the outer food box to allow the animals to freely feed. The animals were taken out after eating or after 10 minutes.
5) On the fifth day, put the food in the food box and repeat the training of the previous day, twice a day.
6) After the sixth day, randomly select 4 arms, one for each arm; the arm of each of the eight-armed labyrinth is closed, and the animal is placed in the middle of the maze; after 30s, the arm door is opened, allowing the animal to be free in the maze. Activities and ingest the pellets until the animals have eaten all the four arms. If the food has not been eaten after 10 minutes, the experiment is terminated. Train twice a day, with an interval of more than 1h.
The ZS animal behavior software automatically records the following four indicators: 1 working memory errors 2 reference memory errors; 3 total number of arms; 4 test time, that is, the animals eat all the grains The time spent. Â
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