Vitrification cryopreservation technology - successfully solve the problem of cryopreservation and recovery of living tumor tissue, assist in the construction of live tumor sample bank and precise treatment of tumor
The vitrification cryopreservation technology successfully breaks through the problem of low survival rate after cryopreservation of living tumor tissue, so that the frozen tumor cells can preserve the tumor tissue activity for a long time , and the tissue morphology and function can be maintained almost identical to the fresh tumor tissue after tissue resuscitation.
1. Significance of the live tumor tissue sample bank
Living tissue, especially living tumor tissue, has important scientific and clinical application value, and living tissue preservation technology is the basis for realizing these values. The commonly used methods for preservation of living tumor tissues are mainly to establish preservation and cryopreservation of PDX tumor models. The establishment of PDX tumor model preservation is time-consuming and labor-intensive, the procedure is cumbersome and expensive, and after repeated passages, the tumor tissue is prone to variability, tumor tissue heterogeneity is lost, and the value of preservation is lost.
The frozen tissue of the living tumor tissue is stored in a low temperature environment, so that the tissue is temporarily detached from the growth state, and the characteristics of the tumor tissue are preserved. This kind of preservation method is labor-saving, easy to operate, and low in cost. When necessary, the tissue is transplanted and resuscitated, so that it can proliferate in the form of primary tumors, and the original mutation of the tumor is well preserved, and comprehensive levels of tumor information data such as molecules, cells, tissues and bodies can be obtained.
2, existing live tumor tissue cryopreservation technology - low survival rate of tumor tissue after cryopreservation
In the past, the fresh frozen tumor tissue used in the cryopreservation method is similar to the cell cryopreservation. Generally, the program is cooled and frozen. The tissue cryopreservation solution is generally composed of DMSO, serum, and cell culture solution, and needs to be used now. However, due to the indeterminate composition of the serum, the stability of the cryopreservation solution is poor, so that the shelf life of the tissue cryopreservation solution is short, and the storage conditions are also harsh.
When cryopreserved tumor tissue using existing tissue cryopreservation solution, only a very small number of tumor tissues can be resuscitated or passaged in the form of tumor cells, and it is impossible to proliferate in the form of primary tumors, and the original mutation of the tumor is easily lost. Only data information at the molecular level can be obtained. Therefore, the application range of the existing tissue cryopreservation solution is narrow, and the application value and scientific research transformation ability of the tumor tissue sample are reduced.
3, vitrification cryopreservation technology - long-term preservation of tumor tissue activity
Recently, we have succeeded in breaking through the problem of cryopreservation of living tumor tissue by using vitrification technology. The tumor tissue frozen by this technique can preserve the activity of tumor tissue for a long time, and the tissue morphology and function can be maintained almost identical to that of fresh tumor tissue after tissue resuscitation. The specific characteristics of this technology are:
- It can be stored for a long time after cryopreservation ( >10 years).
- Morphological function after resuscitation is consistent with fresh tissue.
- Primary tumor cells can still be isolated after resuscitation.
- The ability of PDX tumor formation before and after cryopreservation was basically unchanged.
- It can replace frozen sections and paraffin to fix tissue.
- Achieve the preservation of DNA , RNA and protein activity.
- Surgical specimens and biopsy tissues are applicable.
4. Application of live tumor tissue sample bank
Preclinical evaluation of precise treatment of tumor
In the past two years, the concept of precision tumor therapy has been deeply rooted in people's minds, and some breakthroughs have been made in immunotherapy and targeted drugs. However, surgery and chemoradiotherapy are still the main treatment methods, and tumor chemotherapy has become a routine treatment. However, due to the great heterogeneity of the tumor, the treatment response of the same chemotherapy regimen may be different among different patients. Therefore, how to evaluate and select the appropriate chemotherapy regimen has always been the main contradiction between clinicians and patients.
Although previous researchers have made some progress in evaluating tumor drugs using primary tumor cells or tissue spheres, both methods require cell separation of tumor tissues. On the one hand, smaller tumors cannot obtain sufficient cells for research. On the other hand, since the isolated tumor cells are detached from the tumor tissue microenvironment, the results of the tests often fail to truly reflect the sensitivity of the tumor to the drug. Therefore, in recent years, research on thin-layer slice culture of tumor tissue has been paid more and more attention in foreign countries. Studies have shown that thin sections of tumor tissue from 0.2-0.3 mm are sufficient to ensure nutrient absorption and material exchange in the medium. On optimized media and special supports, tumor tissue sections can survive for more than 7 days, fully meeting the requirements of in vitro drug screening. Compared with primary tumor cells or tissue spheres, thin-layer tumor tissue sections require less tissue volume and retain intact tumor tissue structure. By in vitro culture combined with chemotherapy drugs, the tumor can be truly and efficiently reflected. Sex. Our previous study indicated that cryopreserved tumor tissue can retain 70-80% of the activity of fresh tissue, and it can be completely recombined with fresh tumor tissue sections by in vitro tumor tissue thin slice culture test, which can be a tumor recurrence. And metastatic patients provide real-time tumor drug susceptibility testing.
In addition, cryopreserved resuscitation of live tumor tissue can also be used to establish a PDX model (human tumor animal model). Due to the high simulation of the biocharacteristics of the patient's tumor, the PDX model is considered to be the best tumor research and treatment evaluation model for Drug development, testing and biomarker identification. The ideal living tumor tissue should be directly derived from the patient. Because the establishment procedure of the PDX model mouse is complicated and cumbersome, and the running cost is high, only a small amount of patient tumor tissue can be utilized. The use of live tumor tissue cryopreservation technology can achieve immediate cryopreservation and resuscitation of tumor tissue, combined with PDX model, can provide an in vivo evaluation model for preclinical evaluation of tumor treatment. Therefore, cryopreservation of living tumor tissue provides a possibility for thin section culture of tumor tissue and establishment of PDX model, and also provides a new idea for precise treatment of tumor.
Precious resources and effective tools for cancer research
Previous tumor tissue samples contain "dead" tumor tissue, such as frozen tissue and tissue wax blocks, which can only provide "static" information such as DNA, RNA and protein. Once the tumor is frozen, it cannot be "resurrected". Unable to get "dynamic" information about the tumor. Due to the great heterogeneity of tumors, and the tumor tissue has been in the process of evolution, static and immobilized information can not truly reflect the changes of tumors. On the other hand, with the rapid development of cancer research, new therapeutic drugs and means emerge in an endless stream, and it is urgent to study and test the "live" tumor tissue. Therefore, the breakthrough of cryopreservation and resuscitation technology of living tumor tissue provides a new breakthrough for cancer research. Resources and means.
Hair Wash Shampoo,Natural Shampoo,Hair Nourishing Shampoo,Ginger Hair Shampoo
Wuxi Keni Daily Cosmetics Co.,Ltd , https://www.wxkenidaily.com