In the early 1980s, British psychologist Morris and his colleagues used rats to search for targets in opaque pools containing water and milk mixtures to study the damage of the hippocampus and other brain regions of rats. The memory and spatial orientation and cognitive abilities have achieved remarkable results. The device is not only novel in concept, reasonable in experimental design, but also simple and practical in method. It is also convenient to observe and record the time, strategy and swimming trajectory of animals hidden in the underwater platform after entering the water, so that the animals can be analyzed and inferred. The ability to learn, remember and spatially recognize. Although the initial experimental subjects were rats, the labyrinth system has since become a classical procedure for assessing spatial learning and memory in rodents, and is widely used in basic and applied research in the fields of neurobiology and pharmacology. It can objectively measure changes in animal spatial memory, working memory, and spatial discrimination. Therefore, this research method quickly attracted the attention of neuroscientists in various countries, and this method is called the Morris water maze method. There are many companies producing Morris water maze abroad, such as TSE in Germany, Sandiegoinstruments in the United States, Biobserve in Germany, Noldus in the Netherlands, etc.; in the late 1980s, Morris water maze images were automatically collected in the early 1990s. And processing system. There have been many Morris water maze manufacturers in China. Beijing Zhongshi Di Chuang Technology is a company specializing in the production of behavioral instruments, but from international to domestic, Morris water maze hardware equipment, software systems, experimental methods, and processing of experimental data. The methods are different. Therefore, from the invention of the Morris water maze method to the present, it has been more than 20 years old, and a set of relatively standard experimental criteria should be developed. Â
Light source: To ensure that there is no light and shadow on the water surface of the pool, it is mainly to avoid the reflection of light on the water surface, so as to avoid the shadow of the light left on the water surface being confused by the software collection system with the shadow of the mouse; if it is a white pool background, you can Use two strips of divergent fluorescent light above the pool to reduce the back of the reflected light on the surface of the water; if it is a black pool background, place four 60-watt bulbs or 25-watt tube lights above the four quadrants of the pool. No need for a particularly powerful lamp. The height of the bulb should be high enough to be captured by the camera. In addition, you can also install a curtain around the pool (the color is generally black, light blue or white), which can block the shadow of light and maintain a certain degree of transparency. At the same time, it can also reduce the presence of people outside the pool or walk around, forming an indefinite spatial reference for the rats. Â
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