The research team of Fudan University, Ji Minbiao, collaborated with Harvard University's Xie Xiaoliang research group and the Massachusetts General Hospital (MGH) Brian Bacskai team to use the new stimulated Raman microscopy imaging technique to detect amyloid plaques with misfolded proteins. Mark-free imaging detection. On November 17, the results were published online in Science-Progress.
In many hypotheses about Alzheimer's disease, the amyloid peptide hypothesis suggests that the Aβ protein in the brain is misfolded, causing a cascade reaction that eventually kills neurons and forms amyloid deposits. As the protein of Alzheimer's disease is misfolded, the molecular frequency changes, resulting in a small range of displacement, so the frequency of the protein after misfolding is set in advance, and it can be compared with normal protein. Distinguish it.
The researchers used stimulated Raman scattering (SRS) microscopy to observe the protein precipitation of Alzheimer's disease and compared the images revealed by stimulated Raman imaging with the addition of markers in the same tissue section. Both have almost the same ability to detect amyloid plaques. At the same time, the researchers compared the results of stimulated Raman imaging and dye-labeled fluorescence imaging in fresh tissue, and found that the two were completely consistent with the detection of plaque morphology. It can be seen that the SRS microscopy technique has the same results as the traditional marking method.
“SRS technology has a wider range of observations, and it can find more abundant tissue morphology and chemical composition information than the labeled objects,†said Ji Minbiao.
For the first time, the research results use stimulated Raman imaging technology to detect protein molecules with different secondary structures, providing a new idea for Alzheimer's disease research. At the same time, this method is also expected to be used to explore other and protein errors. Folding related neurodegenerative diseases, including Parkinson's and Huntington's disease. (Reporter Huang Xin)
Source: Chinese Journal of Science
Generic Name: Palbociclib, Palbociclib
English name: Palbociclib
Chinese chemical name: 6-acetyl-8-cyclopentyl-5-methyl-2-[[5-(piperazin-1-yl)-pyridin-2-yl]
Amino]-8H-pyrido[2,3-d]-pyrimidin-7-one
English chemical name: 6-Acetyl-8-cyclopentyl-5-methyl-2-[[5-(piperazin-1-yl)
pyridin-2-yl]amino]-8H-pyrido[2,3-d]pyrimidin-7-one
Chemical Abstracts (CAS) No.: 571190-30-2
Molecular Structure:
Molecular formula: C24H29N7O2
Molecular weight: 447.54
Cas 571188-59-5,Palbociclib Intermediates,2-Chloro-8-Cyclopentyl
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