Consultation: I have a very high pollution rate after inoculation of mycelium mushroom sticks. Is there any good solution?
Answer: Causes of pollution: 1. Raw materials for culture materials are not fresh. 2. The culture material is not completely sterilized. 3. The inoculation operation was not standardized and was infected by mold. 4. Strains with bacteria. 5. The mycelial phase is not ventilated and the temperature is too high.
Control measures: 1. Raw materials for culture materials should be fresh and free of mildew. 2. Culture material sterilization must be thorough. Sterilization at room temperature is to keep the water boiled for 10 to 12 hours after the water is opened. 3. Strict aseptic procedures should be used for inoculation. 4. When the original species or cultivar is opened, the color or other abnormalities must be carefully selected and eliminated. At the same time, it is necessary to increase the inoculation volume. 5. Disinfection in the bacterium room to ensure the cleanliness of the environment. 6. The bacterium room temperature is controlled between 20 and 24 degrees, not too high or too low. Care should be taken to ventilate without affecting the room temperature and ventilate it once or twice a day for 10 to 20 minutes each time.
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