Customers often ask, “Your product manual says sensitivity of 0.1ppb, detection limit of pork – 0.5ppb; pig liver – 1ppb. Is sensitivity different from detection limit? What do they mean?â€
First we can be sure that the sensitivity of the kit itself is not the same as the detection limit for a particular sample.
Sensitivity is for the "test method" and refers to the minimum amount that can be detected by the method. The sensitivity of the quantitative elisa kit itself can generally be understood as the minimum concentration of the standard concentration in the kit standard except for the “ 0†standard or the minimum standard concentration at which the standard curve is established.
The theory is defined as; in elisa, the optical density value of the "0" standard tube can be determined. Determination of 10 or more "0" standard tube, the average value of X-ray density values minus two standard deviations (the SD), isolated from the standard curve corresponding to an optical density of X - 2SD of The concentration is the sensitivity.
The limit of detection is for the "sample" and usually refers to the minimum amount of detection of the actual sample by the kit product. The theory is defined as: 20 negative (blank) samples are measured according to a reasonable pretreatment method, and the average value X and standard deviation (SD) are calculated. The result obtained by the formula X+3SD is the detection (lower) limit of the sample. . The magnitude of the value is closely related to the sample type, sampling area, and pre-processing method of the sample. The limit of detection can be used as an indicator to evaluate the performance of the elisa kit.
For kit products, the higher the sensitivity, the lower the detection limit. For a particular drug and a specific sample, the ideal state is that the median value of the test range is close to the "excessive value" or "positive value" of the drug in a sample. This can reduce errors and make it easier for customers to use. {ie: When setting the judgment threshold (positive negative), it should fall as far as possible on the slope of the standard curve (slope type is steep), close to IC50. }
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